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Edit: Hidehiko
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cltr_1106_HZ86_HI199_0806.jpx
HI199
• ChR2-EYFP expression around PPN
• WGA-555 in ALM to label ALM-projecting thalamus
• anti-Chat
• injected ChR2 virus at different coordinates, which resulted in different proportion of licking following the light stimulation.
The goal is to identify the brain region which triggers the lick most.
jKS_SvobodaLab
cltr_1105_HZ85_HI198_0802.jpx
HI198
• ChR2-EYFP expression around PPN
• WGA-555 in ALM to label ALM-projecting thalamus
• anti-Chat
• injected ChR2 virus at different coordinates, which resulted in different proportion of licking following the light stimulation.
The goal is to identify the brain region which triggers the lick most.
HI-hsyn-ChR2-in-MB
jKS_SvobodaLab
cltr_1095_HZ73_HI187_7536_0801.jpx
HI187
ChR2::EYFP virus injection at PPN combined with SL1 cre injection at thalamus
WGA-555 is injected at ALM
jKS_SvobodaLab
cltr_1094_HZ72_Susu_4714_0729.jpx
Susu-animal 4714
• FusionRed expression (SL1-GtACR-FusionRed was injected in Pons).
• DiI recording track in ALM
jKS_SvobodaLab
cltr_1093_HZ71_HI181_0727.jpx
HI181
Retrograde labeling of PPN neurons (SL1-hsyn-SIO-GtACR1-KV2.1 in thalamus + AAV-CanKII-cre in midbrain): FusionRed
jKS_SvobodaLab
cltr_1117_HZ89_HI214_9310_0919.jpx
HI214
Expressing GtACR-FusionRed in thalamus projecting MB neurons (CamKII promoter).
Recording tracks in Midbrain with DiI.
HI-CamKII-GtACR
jKS_SvobodaLab
cltr_1116_HZ97_HI213_9288_EDF_0918.jpx
HI213
Expressing GtACR-FusionRed in thalamus projecting MB neurons (hsyn promoter).
Recording tracks in Midbrain with DiI.
HI-hsyn-GtACR
jKS_SvobodaLab
cltr_1115_HZ96_HI212_EDF_0919.jpx
HI212
Expressing GtACR-FusionRed in thalamus projecting MB neurons (hsyn promoter).
Recording tracks in Midbrain with DiI.
HI-CamKII-GtACR
HI-PPN-recording
jKS_SvobodaLab
cltr_1114_HZ88_HI201_EDF_0916.jpx
HI201
• ChR2-EYFP expression around PPN
• WGA-555 in ALM to label ALM-projecting thalamus
• ChR2 virus injected at different coordinates, which resulted in different proportion of licking following the light stimulation. The goal is to identify the brain region which triggers the lick most.
HI-hsyn-ChR2-in-MB
jKS_SvobodaLab
cltr_1113_HZ87_HI200_EDF_0906.jpx
HI200
• ChR2-EYFP expression around PPN
• WGA-555 in ALM to label ALM-projecting thalamus
• ChR2 virus injected at different coordinates, which resulted in different proportion of licking following the light stimulation. The goal is to identify the brain region which triggers the lick most.
HI-hsyn-ChR2-in-MB
jKS_SvobodaLab
cltr_1112_HZ82_HI190_EDF_0913.jpx
HI190
Recordings with ChR2-EYFP viruses
jKS_SvobodaLab
cltr_1127_HZ93_HI204_EDF_0930.jpx
HI204
• AAV-hsyn-ChR2-EYFP injection in midbrain + Fiber optics at VM
• WGA555 injection@ ALM
HI-hsyn-ChR2-in-MB
jKS_SvobodaLab
cltr_1118_HZ90_HI215_EDF_1010.jpx
HI215
• Expressing GtACR-FusionRed in thalamus projecting MB neurons (CamKII promoter).
• Recording tracks in Midbrain with DiI.
HI-CamKII-GtACR
jKS_SvobodaLab
cltr_1167_MR01_MCR2908_1023.jpx
Brain from Margreet
jKS_SvobodaLab
cltr_1129_HZ95_HI208_EDF_1025.jpx
HI208
• AAV-hsyn-ChR2-EYFP injection in midbrain + Fiber optics at VM
• WGA555 injection@ ALM
ChAT labeling
HI-hsyn-ChR2-in-MB
jKS_SvobodaLab
cltr_1128_HZ94_HI207_EDF_1023.jpx
HI207
• AAV-hsyn-ChR2-EYFP injection in midbrain + Fiber optics at VM
• WGA555 injection@ ALM
ChAT labeling
HI-hsyn-ChR2-in-MB
jKS_SvobodaLab
cltr_1132_HZ100_HI223_EDF_1107.jpx
HI223 ANM453624:
Recording in Midbrain with DiI.
GtACR::FusionRed expressed in cholinergic neurons in MB.
HI-ChAT-GtACR
jKS_SvobodaLab
cltr_1131_HZ99_HI211_EDF_1107.jpx
HI211 ANM453385:
Recording in Midbrain with DiI.
GtACR::FusionRed expressed in thalamus projecting midbrain neurons.
HI-CamKII-GtACR
jKS_SvobodaLab
cltr_1130_HZ98_HI209_EDF_1104.jpx
HI209:
Recording in Midbrain with DiI.
ChR2::EYFP expressed in thalamus projecting midbrain neurons.
HI-PPN-recording
jKS_SvobodaLab
cltr_1133_HZ101_HI225_1113.jpx
HI225 ANM453626:
Recording in Midbrain with DiI.
GtACR::FusionRed expressed in cholinergic neurons in MB.
HI-ChAT-GtACR
jKS_SvobodaLab
cltr_1170_HZ111_9725_1202.jpx
Animal number 459725
Red and green retrobeads in midbrain to label inputs to PPN
jKS_SvobodaLab
cltr_1147_HZ108_HI227_EDF_1209.jpx
HI227 : midbrain stimulation experiment
ChR2::EYFP expressed in thalamus projection midbrain neurons
WGA-555 injected in ALM to retrogradely label inputs
HI-hsyn-ChR2-in-MB
jKS_SvobodaLab
cltr_1169_HZ110_9724_beads_1217.jpx
Animal number 459724
Red and Green beads injected into midbrain to label inputs to PPN
jKS_SvobodaLab
cltr_1173_HZ112_SuSu_028_1230.jpx
jKS_SvobodaLab
cltr_1119_HZ91_HI202_1508_EDF_1008aa.jpx
HI202
• AAV-hsyn-ChR2-EYFP injection in midbrain + Fiber optics at VM
• WGA555 injection@ ALM
HI-hsyn-ChR2-in-MB
jKS_SvobodaLab
cltr_1141_HZ102_HI217_0102.jpx
HI217
Recording in Midbrain with DiI.
ChR2::EYFP expressed in thalamus projecting midbrain neurons.
HI-PPN-recording
jKS_SvobodaLab
cltr_1148_HZ109_HI218_0102.jpx
HI218
Recording in Midbrain with DiI.
ChR2::EYFP expressed in thalamus projecting midbrain neurons.
HI-PPN-recording
jKS_SvobodaLab
cltr_1076_HZ81_HI194_0716.jpx
HI194
• GtACR-FusionRed in PPN
• Fiber optics implant at PPN
• Please do anti-Chat
• This is for LOF experiment. I want to know the spread of virus in the midbrain
HI-hsyn-GtACR
jKS_SvobodaLab
cltr_1120_HZ92_HI203_1509_0107.jpx
HI203
• AAV-hsyn-ChR2-EYFP injection in midbrain + Fiber optics at VM
• WGA555 injection@ ALM
HI-hsyn-ChR2-in-MB
jKS_SvobodaLab
cltr_1142_HZ103_HI219_0109b.jpx
HI219
• AAV-hsyn-ChR2-EYFP injection in midbrain + Fiber optics at VM
• WGA555 injection@ ALM
HI-hsyn-ChR2-in-MB
jKS_SvobodaLab
cltr_1145_HZ106_HI222_0110.jpx
HI222
• AAV-hsyn-ChR2-EYFP injection in midbrain + Fiber optics at VM
• WGA555 injection@ ALM
HI-hsyn-ChR2-in-MB
jKS_SvobodaLab
cltr_1144_HZ105_HI220_0110.jpx
HI220
• AAV-hsyn-ChR2-EYFP injection in midbrain + Fiber optics at VM
• WGA555 injection@ ALM
HI-hsyn-ChR2-in-MB
jKS_SvobodaLab
cltr_1143_HZ104_HI210_0113.jpx
HI-PPN-recording
jKS_SvobodaLab
cltr_1146_HZ107_HI226_0114.jpx
HI-hsyn-ChR2-in-MB
jKS_SvobodaLab
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